Hermaphrodite hook ups
This cue is sexually dimorphic, given off only by the hermaphrodite and eliciting a response only in the male.
Males are attracted to, reverse direction of movement frequently, and remain in regions of agar conditioned with hermaphrodites.
From our studies we suggest a form of kinesis that works by attracting males to their mating partners from a distance and functions, once males arrive, in holding attracted males in close proximity. Moreover, other than for the fruit fly (20–22), simple, straightforward model systems in which to study the role of sensory organs and the underpinning genetic and neurologic machinery for processing of such sex-related olfactory cues remain poorly described.
The hermaphrodite vulva is not required for the cue. Here we describe the design and results of several assays that provide evidence for a mate-finding cue in .
It’s an effective evolutionary strategy — and a surprisingly common one among many animals and higher plants.
Males from general sensory mutants Mate-location behavior depends on the ability of the nervous system to integrate incoming mate-specific cues and translate this information into appropriate responses in muscles and other effectors. We also tested vulvaless hermaphrodites for their ability to condition agar and as well both general and specific male sensory mutants for their ability to respond.
Odorant cues have been studied extensively in fruit flies (1), other insects (2), fish (3, 4), and rodents (5), and less in reptiles (6, 7), birds (8), various mammals (9, 10), and primates including humans (11–13). Last, we report cue-detection results from males of several )] strains, males from isolates occur spontaneously at an inconveniently low frequency in the self-progeny of hermaphrodites.
Although mate-finding cues are common throughout Nematoda (14, 15) and have been found in the hermaphroditic trematodes (flukes; refs. To obtain a constant supply of males, isolates were heat shocked and maintained by backcrossing (29, 30).
16–18), it remains odd that no chemical communication has been demonstrated in bringing together mating partners. 19), no assays to address such a mate-finding cue have come into common practice (E. To test males from was verified by use of fructose avoidance and dye-fill assays ( (31). All stocks and animals harvested for upcoming trials were grown on standard 5-cm diameter NG agar plates inoculated with the Bacterial lawns were grown on standard 5-cm diameter agar plates for all trials: three drops of OP50-inoculated LB, spread thinly, with a ≈0.25-centimeter gap between the edge of the lawns and the walls of the plates, to dissuade test animals from leaving trials.